Comparison Study of Conventional Method and Reverse Transcription Polymerase Chain Reaction on Detection of Flavivirus in Mosquitoes 逆转录聚合酶链反应与常规法检测蚊体内黄病毒的比较研究
Methods: Bone Marrow cells from 20 cases of CML in treatment were assayed with I-FISH, conventional cytogenetic G-banding ( CCG) analysis and reverse transcription polymerase chain reaction ( RT-PCR) through which the tumor load was monitored in a quantitative way. 方法应用I-FISH、常规细胞遗传学(CCG)G显带技术和筑巢式逆转录聚合酶链式反应(RT-PCR)技术对20例治疗中CML患者骨髓中的肿瘤负荷进行检测。
The longest open reading frame in the PTI gene, which contains conventional 'TATA' and 'CCAAT' transcription control elements, potentially encode a peptide of 213 amino acids. PTI基因翻译起点上游具有‘TATA’和‘CCAAT’等转录控制元件,其包含的最大阅读框架能编码一个213aa的多肽。
Histone variants are the special chromosome histone types required for chromatin assembly at specific chromosome region, and it can replace the conventional histone via chromatin remodeling to exercise its function to regulate gene transcription under epigenetic level. 组蛋白变体是特殊状态下染色体组装所需的组蛋白类型,一般通过替换常规组蛋白导致染色体重塑进入核小体,能在表观遗传学水平调控基因的转录。